Resources results for "methly h4 lys20"

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Epigenetic Writers and Erasers of Histones H2A, H2B, and H4

Interact with this diagram showing the epigenetic writers and erasers of histone H2A, histone H2B, and Histone H4, including the involvement of acetyltransferases, deacetylases, methyltransferases, demethylases and kinases.

Histone Modification Table

The Histone Modification Table provides a referenced list of many known histone modifications, associated modifying enzymes, and proposed functions.

ChIP Validated Antibodies and Primer Pairs

SimpleChIP Kits and Antibodies validated in-house by our antibody development scientists correlated to positive and negative control primers.

Kinase-Disease Associations

Kinase-disease associations table showing kinases, kinase groups, disease types, and the molecular biology basis for disease.

Protocol Compatibility Table for Immune Signaling and Phenotyping by Flow Cytometry

Use our protocol compatibility table to understand which immune signaling and phenotyping antibodies will work together in your multicolor flow cytometry experiment.

CST CUT&RUN Kit: Validated for Flexibility

Maximize your research with our CUT&RUN Kit. Streamline chromatin profiling with our efficient, user-friendly tools designed for deeper, more insightful analyses.

CUT&Tag Frequently Asked Questions

CUT&Tag Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.

CUT&RUN Frequently Asked Questions

CUT&RUN Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.

CUT&RUN Overview

CUT&RUN is a revolutionary technique that lets you see DNA-protein interactions in a whole new way. Identify protein binding sites at high resolution. Click

CUT&Tag: An Introduction

Learn how CUT&Tag cuts down your chromatin profiling experiment time. You’ll get the same great data with a fraction of the DNA library prep time and improved signal-to-noise.

Neurodegeneration Drug Development

Streamline your neurodegeneration therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.

CUT&Tag DNA Library Yield: What to Do if Yours Is Too Low to Detect with an Agilent Bioanalyzer or TapeStation System

CUT&Tag delivers quality NGS data even if the signal from the Bioanalyzer or TapeStation System is low and the DNA library yield is low.

Chromatin Immunoprecipitation Workflow Solutions for ChIP-qPCR and ChIP-seq

Uses of Intracellular Flow Cytometry

Learn more about how intracellular flow cytometry can be used to measure unique signaling events to do with apoptosis, pluripotency status and epigenetic activity.

CUT&RUN Kit Protocol

Fostering mentorship, application-based learning, and a love of science are all part of the mission of the CST internship program. Students interning with us are partnered with CST Mentors.

CUT&Tag Kit Protocol

Stem Cell and Lineage Markers

The ESC and Lineage Markers diagram provides an overview of ESC differentiation along lineage-specific pathways and links to products from CST.

CUT&Tag Troubleshooting Guide

A troubleshooting guide for CUT&Tag–a ChIP-seq alternative with lower sample requirements, lower sequencing cost, and improved signal-to-noise for profiling chromatin and protein-DNA interactions.

Resources

The expected yield of an amplified CUT&Tag DNA library depends on the DNA quantification system used.

If you are using a NanoDrop Spectrophotometer or QIAxpert System, the typical reading is between 10-20 ng/µL for histone targets and 5-12 ng/µL for non-histone targets. If the library concentration is lower than 3 ng/µL with the NanoDrop or QIAxpert System, please refer to the CUT&Tag Troubleshooting Guide before sequencing your samples.
If you are using a Qubit Fluorometric Quantification System or PicoGreen dsDNA Assay, the typical reading is between 3-10 ng/µL for histone targets and could be lower than 1 ng/µL for non-histone targets.

However, low yield doesn't necessarily indicate a failure of the CUT&Tag experiment. We suggest continuing with next-generation sequencing (NGS) if the Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #…