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8726
Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate)
WB & IP Reagents
Monoclonal Antibody

Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) #8726

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Immunoprecipitation Image 1 - Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate)

Immunoprecipitation of HeLa cell lysates using EGF Receptor (D38B1) XP® Rabbit mAb (Magnetic Bead Conjugate) #6937 (lane 1) and Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) (lane 2). The western blot was probed using EGF Receptor (D38B1) XP® Rabbit mAb #4267.

To Purchase # 8726S
Product # Size Price
8726S
400 µl N/A

Supporting Data

Concentration 10 mg/ml
SENSITIVITY Endogenous
Source/Isotype Rabbit IgG

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

This Cell Signaling Technology (CST) antibody is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic bead. Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.

Product Usage Information

Directions for Use: Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.

For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution using our 6-Tube Magnetic Separation Rack #7017. Place the tubes containing the beads in the Magnetic Separation Rack and wait 1 to 2 minutes for the solution to clear before carefully removing the supernatant. Remove the tubes from the Magnetic Separation Rack, add new solution and resuspend the beads by gently vortexing or rocking the tube.

Application Dilution
Immunoprecipitation 1:20

Storage

Supplied in PBS Buffer (pH 7.2), 0.1% Tween® 20. Store at 4°C. Do not aliquot the antibodies.

Specificity / Sensitivity

Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.

Background

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

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