Immunoprecipitation of HeLa cell lysates using EGF Receptor (D38B1) XP® Rabbit mAb (Magnetic Bead Conjugate) #6937 (lane 1) and Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) (lane 2). The western blot was probed using EGF Receptor (D38B1) XP® Rabbit mAb #4267.
This Cell Signaling Technology (CST) antibody is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic bead. Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.
Directions for Use: Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.
For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution using our 6-Tube Magnetic Separation Rack #7017. Place the tubes containing the beads in the Magnetic Separation Rack and wait 1 to 2 minutes for the solution to clear before carefully removing the supernatant. Remove the tubes from the Magnetic Separation Rack, add new solution and resuspend the beads by gently vortexing or rocking the tube.
Supplied in PBS Buffer (pH 7.2), 0.1% Tween® 20. Store at 4°C. Do not aliquot the antibodies.
Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.