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48801
SARS-CoV-2 Spike RBD (318-541) Recombinant Protein (8xHis-Tag)
Recombinant Proteins
Protein Control

SARS-CoV-2 Spike RBD (318-541) Recombinant Protein (8xHis-Tag) #48801

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other Image 1 - SARS-CoV-2 Spike RBD (318-541) Recombinant Protein (8xHis-Tag)

Binding kinetics between Human ACE2 (18-652) Recombinant Protein (mFc-Tag) #83986 (immobilized) and

SARS-CoV-2 Spike RBD (318-541) Recombinant Protein (8xHis-Tag) (in solution, at the indicated concentrations). The vertical red line (180 sec) indicates addition of PBS to induce dissociation. Binding was detected with an anti-mouse Fc biosensor. Values on y-axis indicate binding response signals recorded for 5 different concentrations of SARS-CoV-2 Spike RBD (318-541) Recombinant Protein (8xHis-Tag) (62.5, 125, 250, 500, and 1000 nM).

other Image 2 - SARS-CoV-2 Spike RBD (318-541) Recombinant Protein (8xHis-Tag)

Binding kinetics between SARS-CoV-2 Spike Protein (CR3022) Mouse IgG2a mAb #24387 (immobilized) and SARS-CoV-2 Spike RBD (318-541) Recombinant Protein (8xHis-Tag) (in solution, at indicated concentrations). The vertical red line (180 sec) indicates addition of PBS to induce dissociation. Binding was detected with an anti-mouse Fc biosensor. Values on y-axis indicate binding response signals recorded for 5 different concentrations of SARS-CoV-2 Spike RBD (318-541) Recombinant Protein (8xHis-Tag) (31.2, 62.5, 125, 250, and 500 nM).

other Image 3 - SARS-CoV-2 Spike RBD (318-541) Recombinant Protein (8xHis-Tag)

The purity of SARS-CoV-2 Spike RBD (318-541) Recombinant Protein (8xHis-Tag) was determined by densitometry after SDS-PAGE of 2 μg of protein, followed by staining with Coomassie Blue. Purity values were determined from the DTT-reduced samples (+).

Supporting Data

MW (kDa) 34, 68 kDa (non-reduced); 36 kDa (reduced)

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Formulation

Expression Host: Human (HEK293 cells)

Supplied in PBS solution (pH 7.2).

Storage

Stable at -80°C for 1 year after receipt. Avoid repeated freeze-thaw cycles.

Product Description

SARS-CoV-2 Spike RBD (318-541) Recombinant Protein (8xHis-Tag) is derived from a recombinant expression construct corresponding to the host receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. The expressed protein contains an 8xHis-Tag at its carboxy terminus.

Purity

98%, determined by SDS-PAGE.

Background

The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is similar to other coronaviruses, and is comprised of four key structural proteins: S, the spike protein, E, the envelope protein, M, the membrane protein, and N, the nucleocapsid protein (2). Coronavirus spike proteins are class I fusion proteins and harbor an ectodomain, a transmembrane domain, and an intracellular tail (3,4). The highly glycosylated ectodomain projects from the viral envelope surface and facilitates attachment and fusion with the host cell plasma membrane. The ectodomain can be further subdivided into host receptor-binding domain (RBD) (S1) and membrane-fusion (S2) subunits, which are produced upon proteolysis by host proteases at S1/S2 and S2’ sites. S1 and S2 subunits remain associated after cleavage and assemble into crown-like homotrimers (2,4). In humans, both SARS-CoV and SARS-CoV-2 spike proteins utilize the angiotensin-converting enzyme 2 (ACE2) protein as a receptor for cellular entry (5-7). Spike protein subunits represent a key antigenic feature of coronavirus virions, and therefore represent an important target of vaccines, novel therapeutic antibodies, and small-molecule inhibitors (8,9).

  1. Zhou, P. et al. (2020) Nature 579, 270-3.
  2. Tortorici, M.A. and Veesler, D. (2019) Adv Virus Res 105, 93-116.
  3. Li, F. et al. (2006) J Virol 80, 6794-800.
  4. Li, F. (2016) Annu Rev Virol 3, 237-61.
  5. Shang, J. et al. (2020) Nature 581, 221-4.
  6. Wrapp, D. et al. (2020) Science 367, 1260-3.
  7. Yan, R. et al. (2020) Science 367, 1444-8.
  8. Yuan, Y. et al. (2017) Nat Commun 8, 15092.
  9. Amanat, F. and Krammer, F. (2020) Immunity 52, 583-9.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 48801S
Product # Size Price
48801S
100 µg N/A

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