For PTMScan® protocols we recommend making a 5 mg/ml stock solution by resuspending 1.2 mg in 0.24 ml of 20 mM HEPES pH 8.0, aliquot for single use and store at -80°C. See the PTMScan® protocol for further details.
Store lyophilized Lys-C at -20°C. Once reconstituted, the enzyme can be aliquoted and stored at -80°C to help avoid damaging freeze thaw cycles. Lyophilized Lys-C has a shelf life of 1 year at -20°C and solutions are stable for 6 months at -80°C.
Lysl-endopeptidase (Lys-C) hydrolyzes amide and peptide ester bonds on the carboxyl side of lysine residues and hydrolyzes S-aminoethylcysteine residues.
Lysl-endopeptidase (Lys-C) cleaves proteins on the carboxyl side of lysine residues. This makes it a useful tool in protein sequencing and proteomic applications. The enzyme was originally purified from the Gram-negative soil bacteria Achromobacter lyticus (1). PTMScan® Lys-C is produced recombinantly in E. coli. It has been found to work optimally at the temperatures of 30-37°C and pH ranges from 9.0-9.5 but will undergo degradation at temperatures of 50°C and higher. Activity is maintained in 4M urea or 0.1% SDS solutions for up to 6 hours at 30°C. Inhibitors of the enzyme include PMSF, DFP, and TLCK.
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