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97673
PTMScan® Kinase Activation Antibody Sampler Kit
Proteomic Analysis Products
PTMScan

PTMScan® Kinase Activation Antibody Sampler Kit #97673

Citations (0)
The motif logo was generated from a PhosphoScan® LC-MS/MS experiment using 471 nonredundant tryptic peptides derived from Jurkat cells treated with Calyculin A #9902 and pervanadate, and immunoprecipitated using Immunoaffinity beads from PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Kit #12170. This PhosphoSitePlus® (PSP) logo represents the enrichment of an amino acid at a residue position relative to the abundance of that amino acid in the background, in this case, the human proteome. Residues represented at the top of the logo are the most enriched, whereas those below the y-axis occur less frequently in the sample than would be expected in the proteome. The PSP logo reflects the specificity of the antibody for the S*/T*DXE motif. For more information on motif analysis using PSP, please visit www.phosphosite.org.
The motif logo shows the amino acid distributions around the sites recognized by Immunoafiinity beads from PTMScan® Phospho-Akt Substrate Motif Kit #5561.
The motif logo shows the amino acid distributions around the sites recognized by the Immunoaffinity beads from PTMScan® Phospho-AMPK Substrate Motif (LXRXXS*/T*) Kit #5564.
The motif logo shows the amino acid distributions around the sites recognized by Immunoaffinity beads from PTMScan® Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Kit #5565.
Motif analysis was done using all tyrosine phosphopeptides generated from mouse brain and mouse liver tryptic peptides using Immunoaffinity beads from PTMScan® Phospho-Tyrosine Motif (Y*) (P-Tyr-1000) Kit #8803. The motif logo, drawn from 1178 nonredundant sites, shows the amino acid distributions around the sites recognized by Phospho-Tyrosine (P-Tyr-1000) MultiMab Rabbit mAb mix #8954. The motif logo shows that this antibody is a general phospho-tyrosine antibody and does not have amino acid preferences at other positions.
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Product Usage Information

Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase, solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit. An alternate PTMScan® IAP Buffer Plus Detergent #9992 which may reduce nonspecific interactions is available separately. A detailed protocol and Limited Use License allowing the use of the patented PTMScan® method is included with the kit.

Storage

Antibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibodies.

Product Description

PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® enables researchers to isolate, identify and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur (1). For more information on PTMScan® services, please visit www.cellsignal.com/support.

Background

For more information on our PTMScan® Motif Antibody Kits, please visit www.cellsignal.com/support. Background information specific to each component in the sampler kit can be found on the individual webpages. Phospho-PKA Substrate Motif is described on the webpage for PTMScan® Phospho-PKA Substrate Motif (RRXS*/T*) Kit #5565. Phospho-AMPK Substrate Motif is described on the webpage for PTMScan® Phospho-AMPK Substrate Motif (LXRXXS*/T*) Kit #5564. Phospho-Akt Substrate Motif is described on the webpage for PTMScan® Phospho-Akt Substrate Motif mAb 1 (RXXS*/T*) Kit #5561. Phospho-CK2 Substrate Motif is described on the webpage for PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Kit #12170. Phospho-Tyrosine is described on the webpage for PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit #8803.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
AcetylScan is a registered trademark of Cell Signaling Technology, Inc.
MethylScan is a registered trademark of Cell Signaling Technology, Inc.
MultiMab is a registered trademark of Cell Signaling Technology, Inc.
PTMScan is a registered trademark of Cell Signaling Technology, Inc.
UbiScan is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.