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30299
PTMScan® Control Peptides Succinyl-Lysine
Proteomic Analysis Products
Cell Extracts

PTMScan® Control Peptides Succinyl-Lysine #30299

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PTMScan® Control Peptides Succinyl-Lysine: Image 1
Annotated tandem mass spectrum (MS2) of the PTMScan® Control Peptides Succinyl-Lysine component: FFK(succ)FS[R].
PTMScan® Control Peptides Succinyl-Lysine: Image 2
Annotated tandem mass spectrum (MS2) of the PTMScan® Control Peptides Succinyl-Lysine component: IGFAEK(succ)VAA[K].
PTMScan® Control Peptides Succinyl-Lysine: Image 3
Annotated tandem mass spectrum (MS2) of the PTMScan® Control Peptides Succinyl-Lysine component: STVAQLVK(succ)[R].
PTMScan® Control Peptides Succinyl-Lysine: Image 4
Peptides included in the PTMScan® Control Peptides Succinyl-Lysine mix.  All peptides are stable-isotope labeled, designated by bracketed R or K, and contain a succinyl group designated by parentheses.
PTMScan® Control Peptides Succinyl-Lysine: Image 5
Extracted ion chromatograms of PTMScan® Control Peptides Succinyl-Lysine added at supplied concentration (1X at 200 fmol) to mouse liver peptides prior to immunoaffinity enrichment using PTMScan® HS Succinyl-Lysine Motif (Succ-K) Kit #60724.  Desalted peptides were analyzed on Q Exactive mass spectrometer and resolved using a 90 min reversed phase gradient from 7.5% to 32% acetonitrile on a C18 column.  The peak corresponding to the specific Control Peptide is marked with retention time and observed precursor mass, with peak height reported as the normalized level (NL) for each row per panel.

Product Usage Information

Use with Cell Signaling Technology’s PTMScan® kit protocol from the Immunoaffinity Purification (IAP) step.  Because the optimal amount of PTMScan® Control Peptides Succinyl-Lysine for each user’s experiments will depend on unique factors, such as mass spectrometer sensitivity, users may dilute these control peptides as needed.

1. Aliquot PTMScan® Control Peptides Succinyl-Lysine for storage as single-use units at -20°C or proceed to immediate usage.  
2. Resuspend sample peptides in the appropriate buffer and volume, e.g., 1.4 mL of PTMScan® IAP Buffer (1X).
3. Clear sample peptides by centrifugation.
4. Transfer clarified sample peptides to tubes containing IAP beads.
5. Add 10 µL of PTMScan® Control Peptides Succinyl-Lysine to IAP beads and sample peptides and mix well. 
6. Continue with PTMScan® or PTMScan® HS workflows at the 2-hour incubation step.
7. Detect PTMScan® Control Peptides Succinyl-Lysine in the LCMS data file.

Storage

This product is stable for 12 months when stored at -20°C. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

The PTMScan® Control Peptides Succinyl-Lysine enable quality control of immunoaffinity enrichment performance using PTMScan® or PTMScan® HS workflows.  These synthetic peptides contain a specific post-translational modification (PTM) that can be enriched by the associated PTMScan® or PTMScan® HS immunoaffinity purification (IAP) beads, as well as a stable heavy isotope that can be distinguished from endogenous peptides by the mass spectrometer.  
CAS 75-05-8, 76-05-1

Background

Lysine is subject to a wide array of regulatory post-translational modifications due to its positively charged ε-amino group side chain. The most prevalent of these are ubiquitination and acetylation, which are highly conserved among prokaryotes and eukaryotes (1,2). Acyl group transfer from the metabolic intermediates acetyl-, succinyl-, malonyl-, glutaryl-, butyryl-, propionyl-, and crotonyl-CoA all neutralize lysine’s positive charge and confer structural alterations affecting substrate protein function. Lysine acetylation is catalyzed by histone acetyltransferases, HATs, using acetyl-CoA as a cofactor (3,4). Deacylation is mediated by histone deacetylases, HDACs 1-11, and NAD-dependent Sirtuins 1-7. Some sirtuins have little to no deacetylase activity, suggesting that they are better suited for other acyl lysine substrates (5).
Sirt5 is a predominantly mitochondrial desuccinylase and demalonylase (5,6). In the absence of a known succinyltransferase, succinylation is likely driven by the concentration of succinyl-CoA and intracellular pH and is subject to metabolic fluctuations (7,8). Protein succinylation is especially prevalent among mitochondrial metabolic proteins and bacteria, further solidifying the evolutionary link between mitochondria and prokaryotes. It often occurs at lysine residues that are alternatively acetylated or ubiquitinated. More than a thousand lysine succinylation sites were identified on hundreds of proteins, including glutamate dehydrogenase (15 sites), malate dehydrogenase, citrate synthase, carbamoyl phosphate synthase 1, and histone proteins (9).
  1. Liu, Z. et al. (2014) Nucleic Acids Res 42, D531-6.
  2. Lee, S. (2013) Toxicol Res 29, 81-6.
  3. Lin, H. et al. (2012) ACS Chem Biol 7, 947-60.
  4. Zhang, Z. et al. (2011) Nat Chem Biol 7, 58-63.
  5. Du, J. et al. (2011) Science 334, 806-9.
  6. Peng, C. et al. (2011) Mol Cell Proteomics 10, M111.012658.
  7. Rardin, M.J. et al. (2013) Cell Metab 18, 920-33.
  8. Park, J. et al. (2013) Mol Cell 50, 919-30.
  9. Weinert, B.T. et al. (2013) Cell Rep 4, 842-51.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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To Purchase # 30299
Cat. # Size Qty. Price
30299S
100 µl  (10 assays)
N/A

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