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96632
SignalStain® Vivid Purple Peroxidase Substrate Kit
Primary Antibodies

SignalStain® Vivid Purple Peroxidase Substrate Kit #96632

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Immunohistochemical analysis of paraffin embedded human breast carcinoma using E-Cadherin (4A2) Mouse mAb #14472 (purple) and Vimentin (D21H3) XP® Rabbit mAb #5741 (brown). Note: SignalStain® Boost IHC Detection Reagent (HRP, Mouse) #8125 and SignalStain® Vivid Purple Peroxidase Substrate Kit were used to detect E-Cadherin (4A2) Mouse mAb. SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114 and SignalStain® DAB Substrate Kit #8059 were used to detect Vimentin (D21H3) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human melanoma using Sox10 (E6B6I) XP® Rabbit mAb #69661.

Immunohistochemical analysis of paraffin-embedded human melanoma using MLANA/MART-1 (E9Q4O) XP® Rabbit mAb #64718.

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Product Usage Information

1. To prepare the Vivid Purple working solution, to 5 ml SignalStain® Vivid Purple Diluent add 3 drops SignalStain® Vivid Purple Reagent 1, 3 drops SignalStain® Vivid Purple Reagent 2, 3 drops SignalStain® Vivid Purple Reagent 3, and 3 drops SignalStain® Vivid Purple Reagent 4. Mix well before use.

2. After incubation with SignalStain® Boost IHC Detection Reagent or other HRP-based detection systems, wash slides and incubate with the working solution at room temperature for 2-15 minutes. Optimal development time should be determined by the investigator.

3. Wash slides for 5 minutes in water.

4. Counterstain, if desired.

5. Dehydrate, clear and permanently mount in a non-aqueous mounting medium. Do not use recycled alcohols with this product.

Prolonged incubation in alcohol may decrease sensitivity.

Unused working solution is stable up to 14 days if stored at 2-8ºC. Working solution may change color during this time with no impact on quality or intensity of the staining. If a precipitate forms, filter through a 0.45 μm filter.

Appropriate personal protective equipment should be worn when handling Vivid Purple. Dispose of in accordance with local regulations.

Storage:

Substrate kit should be stored at 4ºC, protected from light. Each kit is stable for 12 months upon receipt if the reagents are not combined.

Product Description

The SignalStain® Vivid Purple Peroxidase Substrate Kit contains all of the necessary reagents to prepare a working solution of Vivid Purple for staining tissue sections. The Vivid Purple working solution reacts with peroxidase (HRP) detection systems such as the SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, and HRP, Mouse #8125), yielding a purple reaction product that can provide color contrast in pigmented tissues such as melanoma.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

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