Immunohistochemical analysis of paraffin-embedded normal human brain using TMEM119 (E3E4T) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human cerebellum (left) or colon carcinoma (right) using TMEM119 (E3E4T) Mouse mAb (top) or TMEM119 Rabbit mAb (bottom). These two antibodies detect independent, unique epitopes on human TMEM119. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemical analysis of paraffin-embedded normal human lung using TMEM119 (E3E4T) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using TMEM119 (E3E4T) Mouse mAb (left) compared to concentration-matched Mouse (E5Y6Q) mAb IgG2a Isotype Control #61656 (right).
Immunohistochemical analysis of paraffin-embedded human lymphoma using TMEM119 (E3E4T) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded 293T cell pellet, untransfected (left) or TMEM119-transfected (right), using TMEM119 (E3E4T) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded normal human testis using TMEM119 (E3E4T) Mouse mAb.
|Immunohistochemistry (Paraffin)||1:200 - 1:800|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
|SignalStain® Boost IHC Detection Reagent (HRP, Mouse) #8125||SignalStain® Boost IHC Detection Reagent (AP, Mouse) #31926|
|SignalStain® DAB Substrate Kit #8059||SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713|
|SignalStain® Vivid Purple Peroxidase Substrate Kit #96632|
NOTE: Use of detection reagents other than those specified in this protocol may require further optimization of the primary antibody to account for the different sensitivities of the detection reagents.
posted February 2010
revised June 2020
Protocol Id: 280
TMEM119 (E3E4T) Mouse mAb recognizes endogenous levels of total TMEM119 protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TMEM119 protein.
Transmembrane protein 119 (TMEM119) is a cell-surface protein of unknown function, expressed exclusively by the microglia subset of myeloid and neural cells (1). Iba1+ microglia with both ramified and amoeboid morphologies express TMEM119, while Iba1+ macrophages are TMEM119 negative (2). TMEM119 and other homeostatic genes have been shown to be downregulated in disease-associated microglia (DAM). These DAM microglia are conserved in human and mouse and have been identified in Alzheimer’s disease (AD), amyotrophic lateral sclerosis (ALS), and 5XFAD and APP/PS1 mouse models (3,4). This protein’s specificity as a microglia marker has proven itself important for labeling microglia in healthy tissue as well as deciphering them from infiltrating macrophages and other cells types in neurodegenerative disease models (1,2).
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