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81010
PhosphoPlus® RIP3 (Thr231/Ser232) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® RIP3 (Thr231/Ser232) Antibody Duet #81010

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other Image 1 - PhosphoPlus® RIP3 (Thr231/Ser232) Antibody Duet

Confocal immunofluorescent analysis of L-929 cells, untreated (left), pre-treated with Z-VAD (20 μM, 30 min) followed by treatment with SM-164 (100 nM) and Mouse Tumor Necrosis Factor-α (mTNF-α) #5178 (20 ng/mL, 2.25 hr; center), or pre-treated with Z-VAD followed by treatment with SM-164 and hTNF-α and post-processed with λ-phosphatase (right), using Phospho-RIP3 (Thr231/Ser232) (E7S1R) Rabbit mAb (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

other Image 2 - PhosphoPlus® RIP3 (Thr231/Ser232) Antibody Duet

Immunoprecipitation of RIP3 from L-929 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is RIP3 (D8J3L) Rabbit mAb. Western blot analysis was performed using RIP3 (D8J3L) Rabbit mAb. A conformation-specific secondary antibody was used to avoid cross reactivity with IgG.

other Image 3 - PhosphoPlus® RIP3 (Thr231/Ser232) Antibody Duet

Western blot analysis of L-929 cells, untreated (-), or treated with combinations of the following treatments as indicated: Z-VAD (20 μM, added 30 min prior to other compounds; +), SM-164 (100 nM, 3 hr; +), and mouse TNF-α (20 ng/ml, 3 hr; +), using Phospho-RIP3 (Thr231/Ser232) (E7S1R) Rabbit mAb (upper), RIP3 (D8J3L) Rabbit mAb #15828 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

other Image 4 - PhosphoPlus® RIP3 (Thr231/Ser232) Antibody Duet

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length mouse RIP3 (mRIP3; +), using RIP3 (D8J3L) Rabbit mAb.

other Image 5 - PhosphoPlus® RIP3 (Thr231/Ser232) Antibody Duet

Western blot analysis of extracts from various cell lines using RIP3 (D8J3L) Rabbit mAb.

other Image 6 - PhosphoPlus® RIP3 (Thr231/Ser232) Antibody Duet

Western blot analysis of extracts from wild-type (+) or RIP3 knockout (-) mouse spleen using RIP3 (D8J3L) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were kindly provided by Dr. Junying Yuan, Harvard Medical School, Boston MA.

To Purchase # 81010S
Product # Size Price
81010S
1 Kit N/A

Product Includes Quantity Reactivity MW(kDa) Isotype
Phospho-RIP3 (Thr231/Ser232) (E7S1R) Rabbit mAb 91702 100 µl M 46-62 Rabbit IgG
RIP3 (D8J3L) Rabbit mAb 15828 100 µl M R 46-62 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

The receptor-interacting protein (RIP) family of serine-threonine kinases (RIP, RIP2, RIP3, and RIP4) are important regulators of cellular stress that trigger pro-survival and inflammatory responses through the activation of NF-κB, as well as pro-apoptotic pathways (1). In addition to the kinase domain, RIP contains a death domain responsible for interaction with the death domain receptor Fas and recruitment to TNF-R1 through interaction with TRADD (2,3). RIP-deficient cells show a failure in TNF-mediated NF-κB activation, making the cells more sensitive to apoptosis (4,5). RIP also interacts with TNF-receptor-associated factors (TRAFs) and can recruit IKKs to the TNF-R1 signaling complex via interaction with NEMO, leading to IκB phosphorylation and degradation (6,7). Overexpression of RIP induces both NF-κB activation and apoptosis (2,3). Caspase-8-dependent cleavage of the RIP death domain can trigger the apoptotic activity of RIP (8).

Receptor-interacting protein 3 (RIP3) was originally found to interact with RIP and the TNF receptor complex to induce apoptosis and activation of NF-κB (9,10). It has subsequently been shown that the association between RIP and RIP3 is a key component of a signaling pathway that results in programmed necrosis (necroptosis), a necrotic-like cell death induced by TNF in the presence of caspase inhibitors (11-13). RIP3 is phosphorylated at Ser227 and targets the phosphorylation of mixed lineage kinase domain-like protein (MLKL), which is critical for necroptosis (14). In mice, RIP3 is phosphorylated at Thr231 and Ser232, leading to association with MLKL and necroptosis (15).

  1. Meylan, E. and Tschopp, J. (2005) Trends Biochem Sci 30, 151-9.
  2. Hsu, H. et al. (1996) Immunity 4, 387-96.
  3. Stanger, B.Z. et al. (1995) Cell 81, 513-23.
  4. Ting, A.T. et al. (1996) EMBO J 15, 6189-96.
  5. Kelliher, M.A. et al. (1998) Immunity 8, 297-303.
  6. Devin, A. et al. (2000) Immunity 12, 419-29.
  7. Zhang, S.Q. et al. (2000) Immunity 12, 301-11.
  8. Lin, Y. et al. (1999) Genes Dev 13, 2514-26.
  9. Yu, P.W. et al. (1999) Curr Biol 9, 539-42.
  10. Sun, X. et al. (1999) J Biol Chem 274, 16871-5.
  11. Zhang, D.W. et al. (2009) Science 325, 332-6.
  12. He, S. et al. (2009) Cell 137, 1100-11.
  13. Cho, Y.S. et al. (2009) Cell 137, 1112-23.
  14. Sun, L. et al. (2012) Cell 148, 213-27.
  15. Chen, W. et al. (2013) J Biol Chem 288, 16247-61.

Pathways & Proteins

Explore pathways + proteins related to this product.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
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