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30590
PTEN and PDK1 Antibody Sampler Kit II
Primary Antibodies
Antibody Sampler Kit

PTEN and PDK1 Antibody Sampler Kit II #30590

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other Image 1 - PTEN and PDK1 Antibody Sampler Kit II

Immunohistochemical analysis of paraffin-embedded human colon using PTEN (D4.3) XP® Rabbit mAb.

other Image 2 - PTEN and PDK1 Antibody Sampler Kit II

Western blot analysis of extracts from HeLa and NIH/3T3 cells, using Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb. Membranes were either left untreated (-) or treated with (+) calf intestinal phosphatase (CIP) post Western transfer to verify phospho-specificity of the antibody.

other Image 3 - PTEN and PDK1 Antibody Sampler Kit II

Western blot analysis of extracts from various cell lines using PDK1 (D37A7) Rabbit mAb.

other Image 4 - PTEN and PDK1 Antibody Sampler Kit II

Western blot analysis of extracts from PC3 cells, HCT116 wild-type and HCT116 PDK1 -/- cells using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). (HCT116 wild-type and HCT116 PDK1 -/- cells were kindly provided by Dr. Bert Vogelstein, Johns Hopkins University, Baltimore, MD).

other Image 5 - PTEN and PDK1 Antibody Sampler Kit II

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

other Image 6 - PTEN and PDK1 Antibody Sampler Kit II

Immunohistochemical analysis using PTEN (D4.3) XP® Rabbit mAb on SignalSlide(TM) PTEN IHC Controls #8106 (paraffin-embedded LNCaP (left) and NIH/3T3 (right) cells).

other Image 7 - PTEN and PDK1 Antibody Sampler Kit II

Western blot analysis of extracts from HCT 116 wild-type and HCT 116 PDK1-/- cells using PDK1 (D37A7) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). (HCT 116 wild-type and HCT 116 PDK1-/- cells were kindly provided by Dr. Bert Vogelstein, Johns Hopkins University, Baltimore, MD).

other Image 8 - PTEN and PDK1 Antibody Sampler Kit II

Western blot analysis of extracts from PC3 cells, untreated or λ phosphatase-treated, using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper), PDK1 Antibody #3062 (middle) or Akt Antibody #9272 (lower).

other Image 9 - PTEN and PDK1 Antibody Sampler Kit II

Western blot analysis of extracts from various cell lines using PTEN (D4.3) XP® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

To Purchase # 30590T
Product # Size Price
30590T
1 Kit  (4 x 20 µl) N/A

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
PTEN (D4.3) XP® Rabbit mAb 9188 20 µl
  • WB
  • IP
  • IHC
H M R Mk Dg 54 Rabbit IgG
Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb 9549 20 µl
  • WB
H M R Mk 54 Rabbit IgG
PDK1 (D37A7) Rabbit mAb 5662 20 µl
  • WB
H M R Hm Mk 58-68 Rabbit IgG
Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb 3438 20 µl
  • WB
  • IP
H M R 58 to 68 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The PTEN and PDK1 Antibody Sampler Kit II provides an economical means to evaluate two key enzymes that regulate multiple signaling pathways. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

PTEN (D4.3) XP® Rabbit mAb detects endogenous levels of total PTEN protein. Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb detects endogenous levels of PTEN when phosphorylated at Ser380, Thr382 and Thr383. It may also react with PTEN singly phosphorylated at Ser380. PDK1 (D37A7) Rabbit mAb detects endogenous levels of total PDK1 protein. Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb detects PDK1 only when phosphorylated at Ser241.

Source / Purification

PTEN (D4.3) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues in the carboxy-terminal sequence of human PTEN. Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser380, Thr382 and Thr383 of human PTEN. PDK1 (D37A7) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PDK1 protein. Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser241 of human PDK1.

Background

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).

Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (11,12), including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (13). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation, and apoptosis.

  1. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
  2. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
  3. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
  4. Wan X and Helman LJ (2003) Oncogene 22, 8205–11
  5. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
  6. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
  7. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
  8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
  9. Funamoto, S. et al. (2002) Cell 109, 611-23.
  10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.
  11. Belham, C. et al. (1999) Curr Biol 9, R93-6.
  12. Toker, A. and Newton, A.C. (2000) Cell 103, 185-8.
  13. Williams, M.R. et al. (2000) Curr Biol 10, 439-48.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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