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PhosphoPlus® PFKFB2 (Ser483) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® PFKFB2 (Ser483) Antibody Duet #39527

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Confocal immunofluorescent analysis of LNCaP (high expression, left) and HepG2 (low expression, right) using PFKFB2 (D5I5F) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from serum starved HeLa cells, untreated (-) or treated with insulin (150 nM, 5 min; +) or IGF-1 (100 ng/ml, 5 min; +), using Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb (upper) or PFKFB2 (D7G5R) Rabbit mAb #13045 (lower). The phospho-specificity of Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb is verified by treating the nitrocellulose membranes with calf intestinal alkaline phosphatase (CIP) and λ protein phosphatase (λ PP) following transfer of proteins from the SDS-PAGE gel to the membranes.

Immunoprecipitation of PFKFB2 from Hep G2 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PFKFB2 (D5I5F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PFKFB2 (D5I5F) Rabbit mAb. An anti-rabbit IgG light chain antibody was used as the secondary antibody.

Western blot analysis of extracts from LNCaP, Hep G2, and MCF7 cells using PFKFB2 (D5I5F) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 39527S
Product # Size Price
1 Kit N/A

Product Includes Quantity Reactivity MW(kDa) Isotype
PFKFB2 (D5I5F) Rabbit mAb 13029 100 µl H Mk 55 Rabbit IgG
Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb 13064 100 µl H 55 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.


The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level (1,2). Fructose 2,6-bisphosphate activates phosphofructokinase, a rate-limiting enzyme in glycolysis, by allosteric regulation (1,2). Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (1,2). Research studies indicate that amino acids activate PFKFB2 through Akt-dependent phosphorylation at Ser483 on PFKFB2 (3). In addition, androgen increases the expression of PFKFB2 in prostate cancer cells (4).

  1. Atsumi, T. et al. (2005) Diabetes 54, 3349-57.
  2. Kim, S.G. et al. (2006) J Biol Chem 281, 2939-44.
  3. Novellasdemunt, L. et al. (2013) J Biol Chem 288, 10640-51.
  4. Moon, J.S. et al. (2011) Biochem J 433, 225-33.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.

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