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85741
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit #85741

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Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 1
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated (+) with LPS (100 ng/mL, 6 hr) and Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation), using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 2
Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/mL, 3 hr; +), using IL-1β (3A6) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 3
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of stimulation; +), using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 4
Western blot analysis of extracts from mouse placenta and mouse spleen using PAI-1 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The differential in PAI-1 expression between placenta and spleen is consistent with their reported RNA expression profiles (www.proteinatlas.org), confirming specificity of the antibody for PAI-1.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 5
Western blot analysis of HeLa cells, mock transfected or transfected with mouse RANTES, using RANTES Antibody (Rodent Specific).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 6
Western blot analysis of extracts from RAW 264.7 cells, untreated (-) or treated with LPS #14011 (1 μg/ml, overnight; +), using MCP-1 (E9R7Z) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 7
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 8
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 9
Western blot analysis of extracts from U87, 3T3-L1 and C2C12 cells using MMP-2 (D4O4T) Rabbit mAb.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 10
Western blot analysis of 1 ng recombinant mouse TNF-α #5178 and 1 ng recombinant rat TNF-α using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 11
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated with Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation; +) and LPS (100 ng/mL, 6 hr; +), using IL-1β (3A6) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 12
Western blot analysis of recombinant Mouse His6Interleukin-6 (mHis6IL-6) using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 13
Immunoprecipitation of MMP-2 protein from U87 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MMP-2 (D2O4T) Rabbit mAb. Western blot analysis was performed using MMP-2 (D2O4T) Rabbit mAb.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 14
Immunoprecipitation of TNF-α from Raw 264.7 cell extracts, treated with LPS (100 ng/mL, 6 hr) and Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific). Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) #3678 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as sencondary antibodies.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 15
Western blot analysis of 1 ng recombinant Human Interleukin-1β (hIL-1β) #8900 and 1 ng recombinant Mouse Interleukin-1β (mIL-1β) #5204 using IL-1β (3A6) Mouse mAb.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 16
Immunoprecipitation of IL-6 from Raw 264.7 cell extracts treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of stimulation) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 17
Confocal immunofluorescent analysis of Raw 264.7 cells, treated with LPS (100 ng/mL, 6 hr; left) or untreated (right), using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 18
Immunohistochemical analysis of paraffin-embedded human large intestine (chronic colitis of the colon) using IL-1-β (3A6) Mouse mAb.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 19
Confocal immunofluorescent analysis of Raw 264.7 cells, untreated (left) or treated with LPS (100 ng/ml, 6 hr; right), using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 20
Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of treatment) (green), using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 21
Immunohistochemical analysis of paraffin-embedded human large intestine (ulcerative chronic colitis of the rectum) using IL-1-β (3A6) Mouse mAb.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 22
Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or treated with LPS (100 ng/ml, 6 hr) (green) using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific). All cells were incubated with Brefeldin A #9972 (300 ng/ml, last 3 hr of treatment). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 23
Immunohistochemical analysis of paraffin embedded THP-1 cell pellets, control (left) or LPS-treated (right) using IL-1-β (3A6) Mouse mAb.
To Purchase # 85741T
Cat. # Size Price
85741T
1 Kit  (6 x 20 microliters) N/A

Product Description

The Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides an economical means of detecting multiple components of the SASP in mouse samples. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit detects endogenous levels of its target protein. RANTES Antibody (Rodent Specific) detects transfected levels of rodent RANTES.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with recombinant human IL-1β protein, recombinant mouse TNF-α protein, recombinant mouse IL-6 protein, a synthetic peptide corresponding to residues surrounding Cys75 of mouse MCP-1 protein, or a synthetic peptide corresponding to residues surrounding Pro117 of human MMP-2 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxyl terminus of rodent RANTES protein or corresponding to residues surrounding Gly196 of murine PAI-1 protein. Polyclonal antibodies are purified by peptide affinity chromatography.

Background

Senescence is characterized by stable stress-induced proliferative arrest and resistance to mitogenic stimuli, as well as the secretion of proteins such as cytokines, growth factors, and proteases. These secreted proteins comprise the senescence-associated secretory phenotype (SASP). Senescent cells are thought to accumulate as an organism ages, and contribute to age-related diseases, including cancer, through promotion of inflammation and disruption of normal cellular function (1,2). The composition of the SASP varies, and SASP components can be either beneficial or deleterious in human disease, depending on the context (3).
The Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides a collection of antibodies to various SASP components, including TNF-α, interleukin-6 (IL-6), the multifunctional cytokine IL-1β, the chemokines RANTES/CCL5 and MCP-1, the matrix metalloprotease MMP-2, and the serine-protease inhibitor PAI-1.
  1. Tchkonia, T. et al. (2013) J Clin Invest 123, 966-72.
  2. Sun, Y. et al. (2018) Trends Mol Med 24, 871-885.
  3. Rao, S.G. and Jackson, J.G. (2016) Trends Cancer 2, 676-687.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.
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