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87887
CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free) #87887

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines and tissues using CD206/MRC1 (E6T5J) XP® Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded CT26.WT syngeneic tumor using CD206/MRC1 (E6T5J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded LL/2 syngeneic tumor using CD206/MRC1 (E6T5J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using CD206/MRC1 (E6T5J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mammary tumor using CD206/MRC1 (E6T5J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse colon using CD206/MRC1 (E6T5J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse pancreas using CD206/MRC1 (E6T5J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse testis using CD206/MRC1 (E6T5J) XP® Rabbit mAb (left) or CD206/MRC1 antibody (right). These two antibodies detect independent, unique epitopes on mouse CD206/MRC1. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD206/MRC1 (E6T5J) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse spleen using CD206/MRC1 (E6T5J) XP® Rabbit mAb (green) and DyLight 650 Phalloidin #12956 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 2: CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse liver using CD206/MRC1 (E6T5J) XP® Rabbit mAb (green) and DyLight 650 Phalloidin #12956 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 3: CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse heart using CD206/MRC1 (E6T5J) XP® Rabbit mAb (green) and DyLight 650 Phalloidin #12956 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
To Purchase # 87887
Cat. # Size Qty. Price
87887SF
100 µg N/A

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 190-250
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #24595. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #24595.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

CD206/MRC1 (E6T5J) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD206/MRC1 protein. This antibody recognizes mouse CD206/MRC1 protein and is also reactive with human CD206/MRC1; however, this antibody is not suggested for immunohistochemical analysis of human tissues. Instead, CD206/MRC1 (E2L9N) Rabbit mAb #91992 is recommended for IHC analysis of human tissue samples.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse CD206/MRC1 protein.

Background

The mannose receptor (MR/CLEC13D/CD206/MMR/MRC1/Macrophage mannose receptor 1) is an endocytic receptor expressed by populations of dendritic cells, macrophages, and nonvascular endothelium (1). The mannose receptor is a heavily glycosylated type I transmembrane protein with three types of extracellular domains and a short carboxy-terminal cytoplasmic domain with no apparent signaling motif (2-4). The extracellular portion of the protein is made up of a CR domain, which binds sulfated glycans, an FNII domain, which binds collagens, and eight C-type lectin domains, which bind carbohydrates containing mannose, fucose, or GlcNAc (4-7). The receptor recycles between the plasma membrane and early endosomes (8). Functions include a role in antigen cross-presentation, clearance of endogenous proteins, pathogen detection, and trafficking through lymphatic vessels (9-12).
  1. Martinez-Pomares, L. (2012) J Leukoc Biol 92, 1177-86.
  2. Lennartz, M.R. et al. (1989) J Biol Chem 264, 2385-90.
  3. Wileman, T.E. et al. (1986) Proc Natl Acad Sci U S A 83, 2501-5.
  4. Taylor, M.E. et al. (1990) J Biol Chem 265, 12156-62.
  5. Fiete, D.J. et al. (1998) Proc Natl Acad Sci U S A 95, 2089-93.
  6. Napper, C.E. et al. (2006) Biochem J 395, 579-86.
  7. Fiete, D. et al. (1997) Proc Natl Acad Sci U S A 94, 11256-61.
  8. Tietze, C. et al. (1982) J Cell Biol 92, 417-24.
  9. Burgdorf, S. et al. (2006) J Immunol 176, 6770-6.
  10. Lee, S.J. et al. (2002) Science 295, 1898-901.
  11. Milone, M.C. and Fitzgerald-Bocarsly, P. (1998) J Immunol 161, 2391-9.
  12. Marttila-Ichihara, F. et al. (2008) Blood 112, 64-72.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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