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6916
4EHP (D54C2) Rabbit mAb
Primary Antibodies

4EHP (D54C2) Rabbit mAb #6916

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# Product Name Application Reactivity
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H M R

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 28
Isotype Rabbit 

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

4EHP (D54C2) Rabbit mAb recognizes endogenous levels of total 4EHP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human 4EHP protein.

Background

Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.

A mammalian cap-binding protein related to eIF4E, 4E Homologous Protein (4EHP), was later identified (6). 4EHP does not interact with eIF4G and therefore cannot activate translation (7). Instead, it inhibits the translation of a subset of mRNAs (7).

  1. Sonenberg, N. et al. (1978) Proc Natl Acad Sci U S A 75, 4843-7.
  2. Gingras, A. et al. (1999) Annu. Rev. Biochem. 68, 913-963.
  3. Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871-80.
  4. Pyronnet, S. et al. (1999) EMBO J. 18, 270-9.
  5. Raught, B. et al. (2000) EMBO J. 19, 434-44.
  6. Rom, E. et al. (1998) J Biol Chem 273, 13104-9.
  7. Zuberek, J. et al. (2007) RNA 13, 691-7.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

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