As a last step for fluorescent assays, add enough neat (undiluted) PI/RNase Staining Solution to submerge cells or samples. Incubate for 15 minutes at room temperature, protected from light before analysis.
Supplied in aqueous buffered solution containing less than 0.09% sodium azide. As with all intercalating DNA dyes, take all necessary safety precautions when using.
Store at 4°C protected from light, do not aliquot or dilute. The solution is stable for 12 months.
PI = 10ug/ml
RNase = 300 ug/ml
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