Confocal immunofluorescent analysis of mouse cerebellum using AQP4 (D1F8E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #30103 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of mouse colon using AQP4 (D1F8E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of mouse kidney using AQP4 (D1F8E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
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This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct immunofluorescent analysis in mouse tissues. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated AQP4 (D1F8E) XP® Rabbit mAb #59678.
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Cover sections with 4% formaldehyde dilute in 1X PBS.
NOTE: Formaldehyde is toxic, use only in fume hood.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
posted November 2006
revised November 2013
Protocol Id: 222
AQP4 (D1F8E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total AQP4 protein.
Human, Mouse, Rat
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human AQP4 protein.
Aquaporins (AQP) are integral membrane proteins that serve as channels in the transfer of water and small solutes across the membrane. There are 13 isoforms of AQP that express in different types of cells and tissues (1,2). AQP1 is found in blood vessels, kidney, eye, and ear. AQP2 is found in the kidney, and it has been shown that the lack of AQP2 results in diabetes (1,3). AQP4 is present in the brain, where it is enriched in astrocytes (1,2,4). AQP5 is found in the salivary and lacrimal gland, AQP6 in intracellular vesicles in the kidney, AQP7 in adipocytes, AQP8 in kidney, testis, and liver, AQP9 is present in liver and leukocytes and AQP10-11 in the intestine (1,3,4). AQPs are essential for the function of cells and organs. It has been shown that AQP1 and AQP4 regulate the water homeostasis in astrocytes, preventing cerebral edema caused by solute imbalance (5). Several studies have shown the involvement of AQPs in the development of inflammatory processes, including cells of innate and adaptive immunity (6,7).
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