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10451
Importazole
Activators & Inhibitors
Chemical Modulators

Importazole #10451

Citations (0)
Chemical structure of Importazole.

Product Usage Information

Importazole is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 1.05 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage

Store lyophilized at room temperature, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 318.4 g/mol
Purity >97%
Molecular Formula C20H22N4
CAS 662163-81-7
Solubility Soluble in DMSO at 30 mg/mL.

Background

Importazole is a cell-permeable, specific inhibitor of importin-β-mediated nuclear import (1). Nuclear import through importin β1 association with adaptor importin α is an essential component of the classical nuclear localization signal (NLS) pathway. Following recognition of the cargo target NLS by importin α, the cargo:importin α:importin β1 complex is transported across the nuclear pore complex into the nucleus, where it dissociates by binding the small, GTP-bound GTPase Ran (2). Importazole blocks importin-β-mediated nuclear import by specifically inhibiting importin-β function, likely through altering the interaction between RanGTP and importin-β (1). Inhibition of the RanGTP/importin-β pathway by Importazole in mitotic cells results in multiple mitotic defects, including defects in spindle assembly, chromosome congression, and spindle centering (3). Similar results were seen in mouse oocytes, where Importazole treatment blocks meiosis I (MI) spindle formation and function (4).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 10451
Cat. # Size Qty. Price
10451S
5 mg