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32774
Aphidicolin
Activators & Inhibitors
Chemical Modulators

Aphidicolin #32774

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other Image 1 - Aphidicolin

Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with Aphidicolin (10 μg/ml, 24 hr; +), using Thymidine Kinase 1 (E2H7Z) Rabbit mAb #28755 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

other Image 2 - Aphidicolin

Chemical structure of Aphidicolin.

Product Usage Information

Aphidicolin is supplied as a lyophilized powder. For a 5 mM stock, reconstitute 1 mg of powder in 590 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 338.5 g/mol
Purity

>98%

Molecular Formula

C20H34O4

CAS 38966-21-1
Solubility Soluble in DMSO at 10 mg/ml or ethanol at 1 mg/ml.

Background

Aphidicolin is a tetracyclic diterpenoid first produced by the fungus Cephalosporium aphidicola. This antibiotic is a potent inhibitor of cellular deoxyribonucleic acid (DNA) synthesis by targeting α, ε, and δ DNA polymerases in eukaryotic cells lines with little to no effect on β or γ DNA polymerases (1,2). Studies have shown that Aphidicolin specifically binds to α DNA polymerase, resulting in the formation of a pol α-DNA-aphidicolin ternary complex that blocks DNA replication. The uses for this compound include synchronized cells at the G1/S boundary and increased gene amplification (3-6).

  1. Longiaru, M. et al. (1979) Nucleic Acids Res 6, 3369-86.
  2. Syväoja, J. et al. (1990) Proc Natl Acad Sci U S A 87, 6664-8.
  3. Dinter-Gottlieb, G. and Kaufmann, G. (1983) J Biol Chem 258, 3809-12.
  4. Sheaff, R. et al. (1991) Biochemistry 30, 8590-7.
  5. Kota, K.P. et al. (2012) Viruses 4, 1865-77.
  6. Yin, D.X. and Schimke, R.T. (1996) Proc Natl Acad Sci U S A 93, 3394-8.
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 32774S
Product # Size Price
32774S
1 mg N/A

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