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75073
A 83-01
Activators & Inhibitors
Chemical Modulators

A 83-01 #75073

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Western blot analysis of extracts from HT-1080 cells, untreated (-) or treated with Human Transforming Growth Factor β1 (hTGF-β1) #8915 (10 ng/ml, 30 min; +) with or without pretreating the cells with A 83-01 (1 μM, 1 hr; +), using Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb #8828 (upper), Smad2/3 (D7G7) XP® Rabbit mAb #8685 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Chemical structure of A 83-01.

Product Usage Information

A 83-01 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 1.18 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 2 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight: 421.5 g/mol

Purity: >98%

Molecular Formula: C25H19N5S

CAS: 909910-43-6

Solubility: Soluble in DMSO at 20 mg/ml.

Background

A 83-01 is a potent and selective inhibitor of TGF-β type I receptor ALK5 kinase, activin type IB receptor ALK4, and nodal type I receptor ALK7 with an IC50 value of 12, 45, and 7.5 nM, respectively (1). This small molecule has also been shown to inhibit Smad2/3 phosphorylation along with TGF-β-induced growth inhibition and induction of Twist and Wnt3 (1,2). A 83-01 can be used to stabilize rat induced pluripotent stem cell (riPSC) cultures by inhibiting spontaneous differentiation and increasing the clonal expansion efficiency (3).

  1. Tojo, M. et al. (2005) Cancer Sci 96, 791-800.
  2. Wu, Y. et al. (2017) Breast Cancer Res Treat 163, 449-60.
  3. Li, W. et al. (2009) Cell Stem Cell 4, 16-9.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

To Purchase # 75073S
Product # Size Price
75073S
5 mg N/A

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