| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| VEGF-A (E9X8Q) Rabbit mAb | 50661 | 20 µl | 16, 20, 23, 26 kDa | Rabbit IgG |
| VEGF Receptor 2 (D5B1) Rabbit mAb | 9698 | 20 µl | 210, 230 kDa | Rabbit IgG |
| Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb | 2478 | 20 µl | 230 kDa | Rabbit IgG |
| PDGF Receptor β (28E1) Rabbit mAb | 3169 | 20 µl | 190 kDa | Rabbit IgG |
| Phospho-PDGF Receptor β (Tyr751) (88H8) Mouse mAb | 3166 | 20 µl | 190 kDa | Mouse IgG2b |
| FGF Receptor 1 (D8E4) XP® Rabbit mAb | 9740 | 20 µl | 92 , 120, 145 kDa | Rabbit IgG |
| Phospho-FGF Receptor 1 (Tyr653/654) (D4X3D) Rabbit mAb | 52928 | 20 µl | 120, 145 kDa | Rabbit IgG |
| Phospho-Tie2 (Tyr992) Antibody | 4221 | 20 µl | 160 kDa | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat | |
| Tie2 (D9D10) Rabbit mAb | 7403 | 20 µl | 160 kDa | Rabbit IgG |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Angiogenesis Receptor Tyrosine Kinase Antibody Sampler Kit provides an economical means of detecting the activation of VEGF receptor 2, FGF receptor 1, PDGF receptor beta, Tie2, and VEGF-A using phospho-specific and control antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
Angiogenesis is defined as the physiological process by which new blood vessels are formed from pre-existing blood vessels. It is a critical process that enables specific physiological conditions in healthy adults, including development, skeletal muscle hypertrophy, and wound healing (1,2). Angiogenesis can be aberrantly activated to generate new blood vessels during pathological conditions such as cancer, neovascular disorders, and chronic inflammation. Angiogenesis is a complicated process regulated by multiple mechanisms and pathways (3). VEGFR2 is a member of the family of receptor tyrosine kinases (RTKs) mainly located in endothelial cells and is a major player in angiogenesis. VEGF-VEGFR2 ligand-receptor activation is the key signaling pathway for angiogenesis activation (4,5). Multiple non-VEGF RTK activations can replace VEGFR to promote angiogenesis (6,7), including PDGFR (9), FGFR (8), and Tie2 (10). These RTKs are targets for an antiangiogenic based disease treatment (11,12).
- Malapelle, U. and Rossi, A. (2019) Expert Opin Emerg Drugs 24, 71-81.
- Chung, A.S. et al. (2010) Nat Rev Cancer 10, 505-14.
- Jain, R.K. (2003) Nat Med 9, 685-93.
- Leung, D.W. et al. (1989) Science 246, 1306-9.
- Jeltsch, M. et al. (2013) Cold Spring Harb Perspect Biol 5, a009183. doi: 10.1101/cshperspect.a009183.
- Zhao, Y. and Adjei, A.A. (2015) Oncologist 20, 660-73.
- Vimalraj, S. (2022) Int J Biol Macromol 221, 1428-1438.
- Lieu, C. et al. (2011) Clin Cancer Res 17, 6130-9.
- Hellberg, C. et al. (2010) Recent Results Cancer Res 180, 103-14.
- Duran, C.L. et al. (2021) Cancers (Basel) 13, 5730. doi: 10.3390/cancers13225730.
- Ramjiawan, R.R. et al. (2017) Angiogenesis 20, 185-204.
- Socinski, M.A. (2011) Cancer Treat Rev 37, 611-7.
Background References
Trademarks and Patents
Limited Uses
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